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KULTUR BAKTERI-ISOLASI DAN INOKULASI-

-RENITA YULIANA-

KULTUR BAKTERI

• Used to grow bacteria

• Enrich the numbers of bacteria

• Select for certain bacteria and

suppress others

• Differentiate among differentkinds of bacteria

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PURE CULTURE

• Mikrobia yang dipelajari harus dalam bentuk

kultur murni

• Strain : kumpulan sel kultur murni yang

berasal dari satu sel tunggal

• Untuk memperoleh kultur murni maka harus

bekerja dengan Teknik Aseptis

• Semua bahan dan alat yang digunakanharus steril

• Isolation

• Menyuburkan bakteri

• To create antigens for laboratory

•Uji Sensibilitas

• Uji Aktivitas Antibakteri

• Estimate viable counts

• Maintain stock culture

PURPOSE OF CULTURING

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METHODS OF ISOLATION OF

PURE CULTURE

• SURFACE PLATING

• ENRICHMENT

MEDIUM

• SELECTIVE MEDIUM

• INDICATOR MEDIUM

METHODS TO ISOLATE THEBACTERIA

• STREAK CULTURE

• POUR PLATE

• SPREAD PLATE

•

STAB• LIQUID CULTURE

• SPECIAL METHODS FOR ANAEROBIC

CULTURES

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STREAK PLATE METHOD

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SLANT OBSERVATION

STAB CULTURE

• Puncturing sutable medium such as

nutrient agar, gelatin

• Observe gelatin liquefaction

• Preserving the stock culture

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LIQUID CULTURING

Liquid cultures are done in:

• Tubes

• Bottles

• Flasks (labu)

• Blood culture

• Water Analysis

BROTH MEDIUM

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LIQUID MEDIA• Easiest to prepare and use

• Good for growing quantities of

microbes needed for analysis of

experiments

• Unless inoculated with pure culture

cannot separate different

organisms

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HOW TO INOCULATE A

CULTURE PLATE

• Plate : providege large surface

for isolation and observation of

colonies

• Using sterile loop or sterile

swab streak your sample on the

petri plate

•

Important let your sterilized loopcool before you pickup your

sample

COLONIES  – MAKE AOBSERVATION

• SHAPE

• SIZE

• ELEVATION

•EDGE

• SURFACE

• OPACITY

• CONSISTENCY

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SWEEP PLATE METHOD

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 ANAEROBIC BACTERIALISOLATION

• Desicator 

• Candle Jar 

• Anaerobic Jar 

• Gas Pack

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TERIMAKASIH