MBC - MOLECULAR BIOTECHNOLOGY CENTER

Mid Term Event – 23 Oct 2013 Panel Discussion – Global Trends in Heathcare and local behaviours

UNIVERSITY OF TURIN - Overview

Agraria, Veterinaria

Medicina e Chirurgia "S.Luigi Gonzaga"

SUISM

SAA

Medicina e Chirurgia Economia

Biotecnologie

Farmacia, Scianze MFN

Psicologia, Giurisprudenza, Lettere e Filosofia, Lingue e Lett. Straniere, Scienze Politiche, Scienze della Formazione SUISS ….

University of Turin was founded in 1404 � 7 University Schools � 3 Centers of Excellence � 27 University Departments � 70.000 students � 1.800 PhD� 4.000 Professors, Researchers

and staff personnel � Incubator

� Technology Transfer Office �

120 Patents (yrs 1999-2013)

Psicologia,Informatica

SUISM

Scienze MFN

Coupled with Interfaculty Biotechnology School

Launched in September 2006 More of 200 scientists

5 main Scientific Area of Interest: � Inflammatory diseases � Oncogenesis and tumor immunology � Cardiac Hypertrophy in heart failure � Stem cells � Bioinformatics

MBC - MOLECULAR BIOTECHNOLOGY CENTEROverview

Main activities: � Animal House for Mice (10.000) and Rabbits (15) � Generation of Transgenic and Knockout Mice � Production and purification of recombinant proteins in

Prokaryotes and Eukaryotes � Production and characterization of monoclonal and

polyclonal antibodies

MBC - MOLECULAR BIOTECHNOLOGY CENTERResearch streams

Stem Cells Tumour Immunology Molecular Imaging

Cardiovascular Oncogenesis Inflammation Bioinformatics

Silvio AIME Full Prof. of General Chemistry Fiorella ALTRUDA Full Prof. of Molecular Genetics Laura BARBERIS Research Scientist of Molecular Biology Mara BRANCACCIO Research Scientist of Cell Biology Benedetta BUSSOLATI Associated Prof. of Pharmacology Sara CABODI Research Scientist of Cell Biology Enzo CALAUTTI Telethon Scientist Giovanni CAMUSSI Full Prof. of Nephrology Federica CAVALLO Associated Prof. of Immunology Paola DEFILIPPI Full Prof. of Cell Biology Ferdinando DI CUNTO Associated Prof. of Molecular Biology Guido FORNI Full Prof. of Immunology Mario GIACOBINI Research Scientist of Informatics Jorg HAMM Lagrange Fellow Emilio HIRSCH Full Prof. of Molecular Genetics Valeria POLI Full Prof. of Molecular Biology Paolo PROVERO Research Scientist of Bioinformatics Francesco RETTA Associated Prof. of Cell Biology Lorenzo SILENGO Full Prof. of Molecular Biology Guido TARONE Full Prof. of Cell Biology Enzo TERRENO Associated Prof. of General Chemistry Emanuela TOLOSANO Associated Prof. of Molecular Genetics Emilia TURCO Associated Prof. of Genetics

Center of Excellence of Molecular Imaging Center for in vivo Functional Genomics Incubator of Ideas

MBC - MOLECULAR BIOTECHNOLOGY CENTERStaff Scientists

Funding from: � European Community� Italian Association for Cancer Research (AIRC) � Telethon for Genetic Diseases � Human Frontieres Science Project � FIRB (Italian Ministry of University and Research) � PRIN (Italian Ministry of University and Research) � Italian Ministry of Health.

MBC - MOLECULAR BIOTECHNOLOGY CENTERResearch Grants and Contracts

RESEARCH GRANTS

� Bracco � Serono � Glaxo SmithKline � Fresenius � BiPCa � RiboVax Bitechnologies

Bracco

RESEARCH CONTRACTS WITH INDUSTRY � NiCox Research Institute � CureTech� IrX Therapuetics � Agennix Inc � Nanogen � Bioline

Foundation Clinical Industrial Research Park was founded to promote the technological transfer of know-how and the results acquired in academic research activities in Medicine, Biomedical Engineering and Bio-Engineering fields.

Foundation CLINICAL INDUSTRIAL RESEARCH PARK

FOUNDERS:

� A new project to extend facilities

� This will be one of the pillar of the knowledge-based next generation hospital, were customized medicine meets patients needs.

MBC - MOLECULAR BIOTECHNOLOGY CENTEROngoing Projects – 1/2

The new building will host: � Inter-academy department by UNITO and POLITO � Large scale facility in Magnetic Resonance � Start ups � Joint labs accademy – industry

Research streams: � Regenerative Medicine � Metabolic-cardiovascular platform

BIOTECHNOLOGY INCUBATOR

Rendering of new building.

� 20.000sqm hosting equipped labs and scientific instruments � Work in progress to build 10.000sqm by 31-Dec-2015

� Financial support of Regione Piemonte and Compagnia di San Paolo

� Contributors:

MBC - MOLECULAR BIOTECHNOLOGY CENTEROngoing Projects – 2/2

Planimetry of new building.

Where the building will be located. Section.

3D view.

Thank you for the attention

BACK UP

ONCOGENESIS AND TUMOR IMMUNOLOGY

B. Bussolati, S. Cabodi, G. Camussi, F. Cavallo, P. Defilippi, G. Forni, V. Poli, F. Retta.

Buttiglieri S, Deregibus MC, Bravo S, Cassoni P, Chiarle R, Bussolati B, Camussi G. Role of Pax2 in apoptosis resistance and proinvasive phenotype of Kaposi's sarcoma cells. J Biol Chem. 2004; 279: 4136-43. Bussolati B, Deambrosis I, Russo S, Deregibus MC, Camussi G. Altered angiogenesis and survival in human tumor-derived endothelial cells. FASEB J. 2003; 17: 1159-61. Cabodi S, Tinnirello A, Di Stefano P, Bisaro B, Ambrosino E, Castellano I, Sapino A, Arisio R, Cavallo F, Forni G, Glukhova M, Silengo L, Altruda F, Turco E, Tarone G, Defilippi P. p130Cas as a new regulator of mammary epithelial cell proliferation, survival, and HER2-neu oncogene-dependent breast tumorigenesis. Cancer Res. 2006; 66: 4672-80 Defilippi P, Di Stefano P, Cabodi S. p130Cas: a versatile scaffold in signaling networks. Trends Cell Biol. 2006; 16: 257-63 Nanni P, Nicoletti G, De Giovanni C, Landuzzi L, Di Carlo E, Cavallo F, Pupa SM, Rossi I, Colombo MP, Ricci C, Astolfi A, Musiani P, Forni G, Lollini PL. Combined allogeneic tumor cell vaccination and systemic interleukin 12 prevents mammary carcinogenesis in HER-2/neu transgenic mice. J Exp Med. 2001; 194: 1195-205. Lollini PL, Cavallo F, Nanni P, Forni G. Vaccines for tumour prevention. Nat Rev Cancer. 2006; 6: 204-16.

PUBLICATIONS by SCIENTIFIC AREA OF INTEREST 1/2

CARDIAC HYPERTROPHY IN HEART FAILURE

L. Barberis, M. Brancaccio, E. Hirsch and G. Tarone Brancaccio M, Fratta L, Notte A, Hirsch E, Poulet R, Guazzone S, De Acetis M, Vecchione C, Marino G, Altruda F, Silengo L, Tarone G, Lembo G. Melusin, a muscle-specific integrin beta1-interacting protein, is required to prevent cardiac failure in response to chronic pressure overload. Nat Med. 2003;9: 68-75 Crackower MA, Oudit GY, Kozieradzki I, Sarao R, Sun H, Sasaki T, Hirsch E, Suzuki A, Shioi T, Irie-Sasaki J, Sah R, Cheng HY, Rybin VO, Lembo G, Fratta L, Oliveira-dos-Santos AJ, Benovic JL, Kahn CR, Izumo S, Steinberg SF, Wymann MP, Backx PH, Penninger JM. Regulation of myocardial contractility and cell size by distinct PI3K-PTEN signaling pathways. Cell. 2002; 110: 737-49. Patrucco E, Notte A, Barberis L, Selvetella G, Maffei A, Brancaccio M, Marengo S, Russo G, Azzolino O, Rybalkin SD, Silengo L, Altruda F, Wetzker R, Wymann MP, Lembo G, Hirsch E. PI3Kgamma modulates the cardiac response to chronic pressure overload by distinct kinase-dependent and -independent effects. Cell. 2004; 118: 375-87.

STEM CELLS

B. Bussolati, E. Calautti, G. Camussi, and F. Di Cunto

Herrera MB, Bruno S, Buttiglieri S, Tetta C, Gatti S, Deregibus MC, Bussolati B, Camussi G. Isolation and characterization of a stem cell population from adult human liver. Stem Cells. 2006 Aug 31 Bussolati B, Bruno S, Grange C, Buttiglieri S, Deregibus MC, Cantino D, Camussi G. Isolation of renal progenitor cells from adult human kidney. Am J Pathol. 2005; 166: 545-55. Calautti E, Li J, Saoncella S, Brissette JL, Goetinck PF. Phosphoinositide 3-kinase signaling to Akt promotes keratinocyte differentiation versus death. J Biol Chem. 2005; 280: 32856-65. Camera P, da Silva JS, Griffiths G, Giuffrida MG, Ferrara L, Schubert V, Imarisio S, Silengo L, Dotti CG, Di Cunto F. Citron-N is a neuronal Rho-associated protein involved in Golgi organization through actin cytoskeleton regulation. Nat Cell Biol. 2003; 5: 1071-8.

PUBLICATIONS by SCIENTIFIC AREA OF INTEREST 2/2

BIOINFORMATICS

F. Di Cunto, P. Provero and L. Silengo Pellegrino M, Provero P, Silengo L, Di Cunto F. CLOE: identification of putative functional relationships among genes by comparison of expression profiles between two species. BMC Bioinformatics. 2004; 5: 179. Cora D, Herrmann C, Dieterich C, Di Cunto F, Provero P, Caselle M. Ab initio identification of putative human transcription factor binding sites by comparative genomics. BMC Bioinformatics. 2005 May 2;6:110.