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Parazitologie potravin a parazitologick laboratorn metody

ZS 2010

VII. Giardie jako pvodci parazitz lovka

Prof. MVDr. Betislav Koudela, CSc.stav parazitologie FVLVeterinrn a farmaceutick univerzita Brnokoudelab@vfu.cz

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Upozornn

Tento soubor obsahuje obrzky z knih a internetu, u nich nebylo

dno o souhlas autora i nakladatele. Je uren vhradn pro

intern potebu v rmci ppravy ke zkouce z pedmtu

Parazitologie potravin a parazitologick laboratorn metody.

Jeho en je zakzno.

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Struktura pednky

informace o giardich v mdich

historie giardizy

biologick charakteristika giardi

vznam infekc giardiemi (water-borne giardiosis)

diagnostick a detekn metody

opaten a prevence

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Giardia intestinalis - uit umn

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Giardia a historie

Antony van

Leeuwenhoek

a

Robert Hook

sekret RoyalSociety v Londn

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Dopis Robertu Hookeovi (1681)

Iweigh 160 pounds and have been of the same weight for some

30 years, and ordinarily in the morning I have a well-formed stool;

however, now and then I have had a looseness at intervals of 2, 3,

or 4 weeks when I went to stool 2, 3, or 4 times a day.

My excrement being so thin I was at diverse times persuaded to

examine it. I have generally seen in my excrement many irregular

particles of sundry sizes.

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DopisRobertu Hookeovi (1681).. pokraovn

All lay in a clear transparent medium, wherein I have sometimes

seen animalcules a-moving very prettily; some of em a bit bigger,

others a bit less than a blood globuletheir bodies were

somewhat longer than broad, and their belly, which was flatlike,furnished with sundry little paws, wherewith they made such a stir

in the clear medium and among the globulesthey made a quick

motion with their paws, yet for all that they made but slow

progress.

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Giardia a historie

1859 Vilm Lambl, PrahaCercomonas intestinalis

1881 G.B. Grassi

Megastoma enterica

1915 Stiles

Giardia lamblia

v souasnosti

Giardia intestinalis Giardia duodenalis Lamblia intestinalis Giardia lamblia

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Morfologie Giardia intestinalis

anterolaterln biky

posterolaterln biky

kaudln biky

ventrln bikymediln tlska

adhesivn disk

ventrln lbek

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Morfologie Giardia intestinalis

trofozoit 10-20 6-16 m

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Morfologie Giardia intestinalis

cysta 12-18 10-16 m

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Druhy giardi

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Giardia intestinalispichycen na enterocytech

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Giardia intestinalispichycen na enterocytech

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Giardia intestinalis

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nejastj nevirov prjmov onemocnn

symptomatick giardiza u 200 mil. lid v Asii, Africe a Latinsk

Americe

symptomy asi u 10% nakaench

prevalence v R 1-10% u dosplch, a 32% u dt

inkuban doba: 12-19 dn

akutn giardiza: prjem, mastn vzhled, bez krve,

pocit slabosti, kee, zvracen

malabsorpce

Giardia intestinalispvodce onemocnn

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Giardiza

Penos

fekln orln

kontaminovan voda

kontaminovan

potraviny

zoonotick penos

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70 druh hostitel ze vech d obratlovc

Giardia intestinalis/duodenalis/lamblia

Geneticky odlin skupiny (7-8 assamblages / druh ?) s preferenchostitele

lovk - assamblage A - lidsk i zvec izolty (bobr ale i pes,koka, pevkavci, prase, more aj.)

- assamblage B - lidsk izolty

Giardiza zoonotick potencil

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Giardiza zoonotick potencil

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mikroskopick nlez

duodenln sond

Enterotest

detekce koproantigen ELISA

PCR

Diagnostika giardizy

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Metody detekce cyst giardi

kontaminovan materil: voda, maso, pop. ostatn potraviny

USEPA (U.S.Enviroment ProtectionAgency) Method 1623, UK

Statutory Instruments 1999 No. 1524

filtrace, flokulace, IFAT, morfologie a morfometrie,

imunomagnetick separace,

PCR

epidemiologick studie: PCR detekce cyst ve vod

testovn viability(in vitro excystace, infekce laboratornch

hlodavc, barvic metody, fluorescence, PCR

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Metody devitalizace cyst giardi ve vod a v potravinch

Bottled Water: drink bottled water from a trusted source, and make sure the

bottle seal is intact.

Boiling Water:

heat the water to a rolling boil for at least 1 minute or usechemical disinfection after water has been boiled for 1 minute.

Filtered Water:

water filters are designed to remove various contaminants from

water. Water filters pass water through a filtering element(physical or chemical), and the contaminants are removed or

neutralized. Use a water filter that has an absolute pore size of

at least 1 micron.

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Dal zdroje informac

Volf P., Hork P. a kol.: Paraziti a jejich biologie. Triton 2007,

318 s.

Hui Y. H. et al. Foodborne Diseases Handbook, Marcel Dekker

Inc. 2001, vol. 2, 516 s.

OrtegaY.: Foodborne Parasite. Springer 2006, 289 s.

http://www.wikipedia.org/