CQ040 Aula 1 Introducao 1SEM2014 Wp

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Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia

Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,

UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia


UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQCQ019 Cromatografia Prof. Luiz Pereira Ramos UFPR/PPPgina /34Luiz Ramos, 20131

Universidade Federal do Paran

Departamento de Qumica

Sub-rea de

Qumica Orgnica

Cromatografia e

EspectrometriaCQ 040

Luiz Pereira RamosAna Luiza Lacava Lordelo


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BIBLIOGRAFIA BSICA

1. COLLINS, Carol H.; BRAGA, Gilberto L.; BONATO, Pierina

S., Fundamentos da Cromatografia. Campinas, SP:Unicamp, 2006.

2. CIOLA, R. Introduo cromatografia em fase gasosa.So Paulo, Edgard Blcher, 1973

3. CIOLA, R. Fundamentos da Cromatografia a Lquido deAlto Desempenho (HPLC), 1 Edio, So Paulo, EdgardBlcher, 1998

http://www.quimica.ufpr.br/lramos

CROMATOGRAFIA


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AULA 1

INTRODUO AOS MTODOSCROMATOGRFICOS

DefinioIntroduo ao Princpio CromatogrficoAdsoro e DessoroPrincipais Tipos de Cromatografia


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CONTEDO PROGRAMTICO

Introduo ao princpio cromatogrficoDefinio de termos

Teoria cromatogrficaTipos de cromatografia

Anlise qualitativa e quantitativaCromatografia de fase lquidaCromatografia de fase gasosa

Aplicaes


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DEFINIO

Conjunto de tcnicas de separao cujo princpio dependeda distribuio diferenciada dos componentes de uma mistura

entre duas fases, uma considerada estacionria e a outra, mvel.

Diferenas nas propriedades das fases mvel e estacionriapossibilitam com que os componentes da amostra se desloquem

atravs do material cromatogrfico com velocidades desiguais,gerando a separao.


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ANLISE CROMATOGRFICA

Afinidade Separao


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Tipos d e in teraoEnergia

(kJ/mol)Espcies que in teragem

on-on 250 Somente ons

on-dipolo 15 ons e molculas polares

2 Molculas polares estacionriasDipolo-dipolo0,3 Molculas polares em rotao

London (disperso) 2 Todos os tipos de molculas

Ligao de hidrognio 20 N, O, F; a ligao um tomoH compartilhado


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ABSORO ADSORO

PRINCPIOSCROMATOGRFI

ABSORO ADSORO


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PRINCPIOS CROMATOGRFICOS

9

Partio


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PRINCPIOS CROMATOGRFICOS

Adsoro10


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Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. LuizPereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,

UFPR/PPGQ CQ019 Cromatografia Prof. LuizPereira Ramos, UFPR/PPGQ CQ019Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. LuizPereira Ramos, UFPR/PPGQ CQ019Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. LuizPereira Ramos, UFPR/PPGQ CQ019Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. LuizPereira Ramos, UFPR/PPGQ CQ019 Cromatografia

Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. LuizPereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,


ABSORO ADSORO


ABSORO ADSORO
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ch:1http://images.google.com.br/imgres?imgurl=http://www.nature.com/nchina/2007/070627/images/nchina.2007.117-i1.jpg&imgrefurl=http://www.nature.com/nchina/2007/070627/full/nchina.2007.117.html&usg=__lMQwIIFGHMAcGP4KcGAiIP4Gs3A=&h=182&w=180&sz=22&hl=pt-BR&start=63&um=1&itbs=1&tbnid=FZwzRvtAQzOfqM:&tbnh=101&tbnw=100&prev=/images%3Fq%3Dimmiscible%2Bliquids%26start%3D54%26um%3D1%26hl%3Dpt-BR%26sa%3DN%26rlz%3D1T4WZPA_enBR326BR328%26ndsp%3D18%26tbs%3Disch:1http://images.google.com.br/imgres?imgurl=http://s1.hubimg.com/u/1150464_f260.jpg&imgrefurl=http://hubpages.com/hub/homeschool-curriculum-liquid-layers&usg=__OwSuTA1mtp0byk7_xTziMniRra4=&h=347&w=260&sz=13&hl=pt-BR&start=41&um=1&itbs=1&tbnid=Wu6BI1Sx31tRJM:&tbnh=120&tbnw=90&prev=/images%3Fq%3D%25C3%25ADmmiscible%26start%3D36%26um%3D1%26hl%3Dpt-BR%26sa%3DN%26rlz%3D1T4WZPA_enBR326BR328%26ndsp%3D18%26tbs%3Disch:1http://images.google.com.br/imgres?imgurl=http://www.fabeni.net/fc/fc/dpquim/funil03.jpg&imgrefurl=http://www.fabeni.net/fc/fc/dpquim/inxmatlab.htm&usg=__Bxx-p85Q1qj6eBc-2RDVFXpQ5Hc=&h=1014&w=676&sz=84&hl=pt-BR&start=5&um=1&itbs=1&tbnid=o1x0WNcf-rH7qM:&tbnh=150&tbnw=100&prev=/images%3Fq%3Dextra%25C3%25A7%25C3%25A3o%2Bl%25C3%25ADquido-l%25C3%25ADquido%26um%3D1%26hl%3Dpt-BR%26rlz%3D1T4WZPA_enBR326BR328%26tbs%3Disch:1http://images.google.com.br/imgres?imgurl=http://s1.hubimg.com/u/1150464_f260.jpg&imgrefurl=http://hubpages.com/hub/homeschool-curriculum-liquid-layers&usg=__OwSuTA1mtp0byk7_xTziMniRra4=&h=347&w=260&sz=13&hl=pt-BR&start=41&um=1&itbs=1&tbnid=Wu6BI1Sx31tRJM:&tbnh=120&tbnw=90&prev=/images%3Fq%3D%25C3%25ADmmiscible%26start%3D36%26um%3D1%26hl%3Dpt-BR%26sa%3DN%26rlz%3D1T4WZPA_enBR326BR328%26ndsp%3D18%26tbs%3Disch:1http://images.google.com.br/imgres?imgurl=http://www.fabeni.net/fc/fc/dpquim/funil03.jpg&imgrefurl=http://www.fabeni.net/fc/fc/dpquim/inxmatlab.htm&usg=__Bxx-p85Q1qj6eBc-2RDVFXpQ5Hc=&h=1014&w=676&sz=84&hl=pt-BR&start=5&um=1&itbs=1&tbnid=o1x0WNcf-rH7qM:&tbnh=150&tbnw=100&prev=/images%3Fq%3Dextra%25C3%25A7%25C3%25A3o%2Bl%25C3%25ADquido-l%25C3%25ADquido%26um%3D1%26hl%3Dpt-BR%26rlz%3D1T4WZPA_enBR326BR328%26tbs%3Disch:1http://images.google.com.br/imgres?imgurl=http://s1.hubimg.com/u/1150464_f260.jpg&imgrefurl=http://hubpages.com/hub/homeschool-curriculum-liquid-layers&usg=__OwSuTA1mtp0byk7_xTziMniRra4=&h=347&w=260&sz=13&hl=pt-BR&start=41&um=1&itbs=1&tbnid=Wu6BI1Sx31tRJM:&tbnh=120&tbnw=90&prev=/images%3Fq%3D%25C3%25ADmmiscible%26start%3D36%26um%3D1%26hl%3Dpt-BR%26sa%3DN%26rlz%3D1T4WZPA_enBR326BR328%26ndsp%3D18%26tbs%3Disch:1http://images.google.com.br/imgres?imgurl=http://www.fabeni.net/fc/fc/dpquim/funil03.jpg&imgrefurl=http://www.fabeni.net/fc/fc/dpquim/inxmatlab.htm&usg=__Bxx-p85Q1qj6eBc-2RDVFXpQ5Hc=&h=1014&w=676&sz=84&hl=pt-BR&start=5&um=1&itbs=1&tbnid=o1x0WNcf-rH7qM:&tbnh=150&tbnw=100&prev=/images%3Fq%3Dextra%25C3%25A7%25C3%25A3o%2Bl%25C3%25ADquido-l%25C3%25ADquido%26um%3D1%26hl%3Dpt-BR%26rlz%3D1T4WZPA_enBR326BR328%26tbs%3Disch:1http://images.google.com.br/imgres?imgurl=http://s1.hubimg.com/u/1150464_f260.jpg&imgrefurl=http://hubpages.com/hub/homeschool-curriculum-liquid-layers&usg=__OwSuTA1mtp0byk7_xTziMniRra4=&h=347&w=260&sz=13&hl=pt-BR&start=41&um=1&itbs=1&tbnid=Wu6BI1Sx31tRJM:&tbnh=120&tbnw=90&prev=/images%3Fq%3D%25C3%25ADmmiscible%26start%3D36%26um%3D1%26hl%3Dpt-BR%26sa%3DN%26rlz%3D1T4WZPA_enBR326BR328%26ndsp%3D18%26tbs%3Disch:1http://images.google.com.br/imgres?imgurl=http://s1.hubimg.com/u/1150464_f260.jpg&imgrefurl=http://hubpages.com/hub/homeschool-curriculum-liquid-layers&usg=__OwSuTA1mtp0byk7_xTziMniRra4=&h=347&w=260&sz=13&hl=pt-BR&start=41&um=1&itbs=1&tbnid=Wu6BI1Sx31tRJM:&tbnh=120&tbnw=90&prev=/images%3Fq%3D%25C3%25ADmmiscible%26start%3D36%26um%3D1%26hl%3Dpt-BR%26sa%3DN%26rlz%3D1T4WZPA_enBR326BR328%26ndsp%3D18%26tbs%3Disch:1http://images.google.com.br/imgres?imgurl=http://s1.hubimg.com/u/1150464_f260.jpg&imgrefurl=http://hubpages.com/hub/homeschool-curriculum-liquid-layers&usg=__OwSuTA1mtp0byk7_xTziMniRra4=&h=347&w=260&sz=13&hl=pt-BR&start=41&um=1&itbs=1&tbnid=Wu6BI1Sx31tRJM:&tbnh=120&tbnw=90&prev=/images%3Fq%3D%25C3%25ADmmiscible%26start%3D36%26um%3D1%26hl%3Dpt-BR%26sa%3DN%26rlz%3D1T4WZPA_enBR326BR328%26ndsp%3D18%26tbs%3Disch:1http://images.google.com.br/imgres?imgurl=http://www.nature.com/nchina/2007/070627/images/nchina.2007.117-i1.jpg&imgrefurl=http://www.nature.com/nchina/2007/070627/full/nchina.2007.117.html&usg=__lMQwIIFGHMAcGP4KcGAiIP4Gs3A=&h=182&w=180&sz=22&hl=pt-BR&start=63&um=1&itbs=1&tbnid=FZwzRvtAQzOfqM:&tbnh=101&tbnw=100&prev=/images%3Fq%3Dimmiscible%2Bliquids%26start%3D54%26um%3D1%26hl%3Dpt-BR%26sa%3DN%26rlz%3D1T4WZPA_enBR326BR328%26ndsp%3D18%26tbs%3Disch:1http://images.google.com.br/imgres?imgurl=http://s1.hubimg.com/u/1150464_f260.jpg&imgrefurl=http://hubpages.com/hub/homeschool-curriculum-liquid-layers&usg=__OwSuTA1mtp0byk7_xTziMniRra4=&h=347&w=260&sz=13&hl=pt-BR&start=41&um=1&itbs=1&tbnid=Wu6BI1Sx31tRJM:&tbnh=120&tbnw=90&prev=/images%3Fq%3D%25C3%25ADmmiscible%26start%3D36%26um%3D1%26hl%3Dpt-BR%26sa%3DN%26rlz%3D1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ABSORO ADSORO


ABSORO ADSORO


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CROMATOGRAFIA

Planar

Lquida

Lquida

CP

Slida

CCD

Ligada

CCD

em Coluna

Gs

Lquido

CGL

Slido

CGS

Ligada

CGFL

Fluido Supercrtico

Slido

CSS

Ligada

CSFL

Lquida

Lquida

CLL

Slida

CLS

Ligada

CLFL,CTI eCB

CE


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Sigla Nome Tipo de separao

CP papel partilhaCCD camada delgada partilhaCCD-FL camada delgada com fase quimicamente ligada partilha e adsoro

CGL gs-lquido distribuioCGS gs-slido adsoroCGFL gasosa com fase quimicamente ligada adsoroCSS slida com fase mvel super-crtica adsoroCSFL CSS com fase quimicamente ligada adsoro

CLL

liquido-lquido

partilha

CLS lquido-slido adsoroCE excluso permeaoCLFL lquida com fase quimicamente ligada partilha e adsoroCTI troca inica interaes polaresCB bioafinidade bioatividade

TIPOS DE CROMATOGRAFIA


15/34






Planar

Lquida

Lquida

CP

Slida

CCD

Ligada

CCD

em Coluna

Gs

Lquido

CGL

Slido

CGS

Ligada

CGFL

Fluido Supercrtico

Slido

CSS

Ligada

CSFL

Lquida

Lquida

CLL

Slida

CLS

Ligada

CLFL,CTI eCB

CE

CROMATOGRAFIA

P f L i P i R UFPR/PPGQ CQ019 C t fi P f L i P i R


16/34






CROMATOGRAFIA PLANARCromatografia em Papel - Ascendente

cuba cromatogrfica

ascendente

FM



17/34






CROMATOGRAFIA PLANARCromatografia em Papel - Ascendente

FM

cuba cromatogrfica

ascendente

Rf1

Rf2Rf3



18/34






Cromatografia em Papel - Descendente

Rf1

Rf2

Rf3

fasemvl

CROMATOGRAFIA PLANAR

cuba cromatogrfica

descendente



19/34






Cromatografia em Camada Delgada

cuba cromatogrfica cromatoplaca

Rf1

Rf2Rf3

FM

CROMATOGRAFIA PLANAR

Prof Luiz Pereira Ramos UFPR/PPGQ CQ019 Cromatografia Prof Luiz Pereira Ramos
http://c/Users/Journal/Documents/Filmes/TLC%20Separation%20of%20Inks%202009%2009%2030.movhttp://c/Users/Journal/Documents/Filmes/TLC%20Separation%20of%20Inks%202009%2009%2030.movhttp://c/Users/Journal/Documents/Filmes/TLC%20Separation%20of%20Inks%202009%2009%2030.movhttp://c/Users/Journal/Documents/Filmes/TLC%20Separation%20of%20Inks%202009%2009%2030.movhttp://c/Users/Journal/Documents/Filmes/TLC%20Separation%20of%20Inks%202009%2009%2030.movhttp://c/Users/Journal/Documents/Filmes/TLC%20Separation%20of%20Inks%202009%2009%2030.movhttp://c/Users/Journal/Documents/Filmes/TLC%20Separation%20of%20Inks%202009%2009%2030.mov


20/34






Planar

Lquida

Lquida

CP

Slida

CCD

Ligada

CCD

em Coluna

Gs

Lquido

CGL

Slido

CGS

Ligada

CGFL

Fluido Supercrtico

Slido

CSS

Ligada

CSFL

Lquida

Lquida

CLL

Slida

CLS

Ligada

CLFL,CTI eCB

CE

CROMATOGRAFIA



21/34




Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQCQ019 Cromatografia Prof. Luiz Pereira Ramos UFPR/PPPgina /34Luiz Ramos, 201321

Gs de Arraste

Sistema de Tratamentode Dados

Interface

Detector

Injetor

x x

Coluna

Fornox

Cromatografia de fase gasosa

http://c/Users/Journal/Documents/Filmes/Gas%20Chromatography%20GC.movhttp://c/Users/Journal/Documents/Filmes/Gas%20Chromatography%20GC.movhttp://c/Users/Journal/Documents/Filmes/Gas%20Chromatography%20GC.movhttp://c/Users/Journal/Documents/Filmes/Gas%20Chromatography%20GC.movhttp://c/Users/Journal/Documents/Filmes/Gas%20Chromatography%20GC.movhttp://c/Users/Journal/Documents/Filmes/Gas%20Chromatography%20GC.movhttp://c/Users/Journal/Documents/Filmes/Gas%20Chromatography%20GC.mov


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Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQCQ019 Cromatografia Prof. Luiz Pereira Ramos UFPR/PPPgina /34Luiz Ramos, 201322

seringa

colunadetectorSeparao


http://c/Users/Journal/Documents/Figuras/Anima%C3%A7%C3%B5es%20t%C3%A9cnicas/Chromatography.mpghttp://c/Users/Journal/Documents/Figuras/Anima%C3%A7%C3%B5es%20t%C3%A9cnicas/Chromatography.mpghttp://c/Users/Journal/Documents/Figuras/Anima%C3%A7%C3%B5es%20t%C3%A9cnicas/Chromatography.mpghttp://c/Users/Journal/Documents/Figuras/Anima%C3%A7%C3%B5es%20t%C3%A9cnicas/Chromatography.mpghttp://c/Users/Journal/Documents/Figuras/Anima%C3%A7%C3%B5es%20t%C3%A9cnicas/Chromatography.mpghttp://c/Users/Journal/Documents/Figuras/Anima%C3%A7%C3%B5es%20t%C3%A9cnicas/Chromatography.mpghttp://c/Users/Journal/Documents/Figuras/Anima%C3%A7%C3%B5es%20t%C3%A9cnicas/Chromatography.mpg


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UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQCQ019 Cromatografia Prof. Luiz Pereira Ramos UFPR/PPPgina /34Luiz Ramos, 201323

Coluna capilar(Narrowbore)

Colunas empacotadas (convencionais)

Coluna capilar(Megabore)




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UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 CromatografiaProf. LuizPereira Ramos, UFPR/PPGQCQ019 Cromatografia Prof. Luiz Pereira Ramos UFPR/PPPgina /34Luiz Ramos, 201324

Programao de temperatura

http://c/Users/Journal/Documents/Figuras/Anima%C3%A7%C3%B5es%20t%C3%A9cnicas/Gas%20chrom.movhttp://c/Users/Journal/Documents/Figuras/Anima%C3%A7%C3%B5es%20t%C3%A9cnicas/Gas%20chrom.movhttp://c/Users/Journal/Documents/Figuras/Anima%C3%A7%C3%B5es%20t%C3%A9cnicas/Gas%20chrom.movhttp://c/Users/Journal/Documents/Figuras/Anima%C3%A7%C3%B5es%20t%C3%A9cnicas/Gas%20chrom.movhttp://c/Users/Journal/Documents/Figuras/Anima%C3%A7%C3%B5es%20t%C3%A9cnicas/Gas%20chrom.mov


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Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,

UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos,UFPR/PPGQ CQ019 Cromatografia Prof. Luiz Pereira Ramos, UFPR/PPGQ CQ019 Cromatografia P

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